Our biology services span a wide range of capabilities with respect to assay development and miniaturization, validation, optimization and implementation, and finally screening of small molecules and peptides.

 

Our skilled scientists will work with you to provide a cost-effective testing format. We provide method descriptions and data along with detailed reports of results with interpretation and suggestions for further work.

Typical areas in which we provide assay services:

  • Primary screening assays
  • Selectivity assays
  • Biomarkers
  • Cell viability and toxicity

With access to advanced instruments, fit-for-purpose laboratories and professional knowledge, we provide a broad range of customized services to meet the needs of our clients. We deliver high-quality data by applying specific criteria during the process to ensure robustness and reproducibility. Both target-based biochemical and cell-based assay formats are supported.

Capabilities & Screening Strategies

We work with projects across therapy areas and have a proven track record in addressing a wide range of drug target classes, including but not limited to:

  • Kinases
  • Proteases
  • Nuclear hormone receptors
  • GPCRs
  • Protein-protein interactions

One of our most requested assay formats is the alpha-screen, which is employed for a variety of targets such as kinases, protein-protein interaction and GPCRs. Another of our expert areas is optimization of existing assays to industrial standard.

We have strong experience in applying biochemical assays in high concentration formats, which can be used in our fragment-based discovery platform or as a stand-alone assay for testing or screening weak binders.

If desired, we can provide input and recommend strategies for choosing the right assay format with respect to relevance, feasibility, cost and time.

 

Cases & References

Red Glead Discovery provides continuous and flexible support for an LO-project for screening peptide compounds in a cell-based assay. In this project, the client appreciates the flexibility of Red Glead Discovery and the high quality of data delivered in a short time to meet timelines.

 

Labs & Technologies

Red Glead Discovery has modern, well-equipped and fit-for-purpose biochemistry and cell culture laboratories.

Key instruments

  • EnVision® Multilabel Plate Reader (Perkin Elmer)
  • Versatile platform enabling high-quality data from a multitude of assay formats: Absorbance, fluorescence, FRET, luminescence, BRET, TRF, HTRF, fluorescence polarization (FP), AlphaScreen, AlphaLISA, etc. EC50/IC50 values from µM to pM; Supports use of 96–384 well plates.
  • SpectraMax 340PC (Molecular Devices)
  • Agilent Cary 60 UV-Vis spectrophotometer

Expanded capabilities through collaboration with Labjoy

Our partnership with Labjoy, also located at Medicon Village, has further enhanced our biology capabilities, particularly in cell biology and biomarker analysis, e.g. cell isolation and separation from whole blood and tissues, and performance of functional assays such as PBMC cell migration and adhesion assays. Read more (pdf)

 

Contact

If you would like to know more about how to exploit our in vitro biology and assay capabilities, please contact us at:

assays@redglead.com

Or visit us in

Building 403 at Medicon Village in Lund, Sweden

 

Contact

+46 46 460 12 90
+46 46 460 12 91
+46 46 460 12 92
info@redglead.com

Visiting address

Medicon Village
Building 403
Scheelevägen 2
223 63 Lund, Sweden

Shipping address

Red Glead Discovery AB
Medicon Village
Scheelevägen 8
223 63 Lund, Sweden

Postal address

Medicon Village
SE-223 81 Lund, Sweden